Large-scale sequencing trials begin

Journal Article · Fri Dec 07 00:00:00 EST 1990 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.2255904· OSTI ID:5821140

Roberts, L

As genome sequencing gets under way, investigators are grappling not just with new techniques but also with questions about what is acceptable accuracy and when data should be released. Four groups are embarking on projects that could make or break the human genome project. They are setting out to sequence the longest stretches of DNA ever tackled-several million bases each-and to do it faster and cheaper than anyone has before. If these groups can't pull it off, then prospects for knocking off the entire human genome, all 3 billion bases, in 15 years and for $3 billion will look increasingly unlikely. Harvard's Walter Gilbert, is first tackling the genome of Mycoplasma capricolum. At Stanford, David Botstein and Ron Davis are sequencing Saccharomyces cerevisiae. In a collaborative effort, Robert Waterson at Washington University and John Sulston at the Medical Research Council lab in Cambridge, England, have already started on the nematode Caenorhabditis elegans. And in the only longstanding project of the bunch, University of Wisconsin geneticist Fred Blattner is already several hundred kilobases into the Escherichia coli genome.

• OSTI ID: 5821140
• Journal Information: Science (Washington, D.C.); (United States), Vol. 250:4986; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESCHERICHIA COLI
DNA SEQUENCING
GENETIC MAPPING
RESEARCH PROGRAMS
MYCOPLASMA
NEMATODES
SACCHAROMYCES CEREVISIAE
ACCURACY
MAN
ANIMALS
ASCHELMINTHES
BACTERIA
EUMYCOTA
FUNGI
HELMINTHS
MAMMALS
MAPPING
MICROORGANISMS
PLANTS
PRIMATES
SACCHAROMYCES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
YEASTS
550400* - Genetics