Properties of a specific interleukin 1 (IL 1) receptor on human Epstein Barr virus-transformed B lymphocytes. Identity of receptor for IL 1-. cap alpha. and IL 1-. beta
The properties of specific human interleukin 1 (IL 1) receptors on human Epstein Barr virus-transformed B lymphocytes (EBV-B) were studied. Purified human IL 1-..beta.. from a myelomonocytic cell line (THP-1) was labeled with /sup 125/I. Among four EBV-B cell lines tested, a pre-B cell type (VDS-O) specifically bound the highest amount of /sup 125/I-IL 1-..beta... The binding of /sup 125/I-IL 1-..beta.. to VDS-O cells was inhibited by F(ab)'/sub 2/ fragments of anti-human IL 1 and recombinant human IL 1-..cap alpha.., as well as by unlabeled human IL 1-..beta.. but not by recombinant lymphotoxin, recombinant tumor necrosis factor, or phorbol myristic acid, suggesting that IL 1-..cap alpha.. and IL 1-..beta.. bind specifically to the same receptor. The m.w. of IL 1 receptor on human EBV-B cells was estimated to be 60,000 by both the chemical cross-linking method and high pressure liquid chromatography (HPLC). The isoelectric point of solubilized human IL 1 receptor was 7.3 on HPLC chromatofocusing. The evidence of existence of IL 1 receptor on human EBV-B cells additionally supports the hypothesis that IL 1 may be an autocrine signal for these cells.
- Research Organization:
- National Institutes of Health, Frederick, MD
- OSTI ID:
- 5785625
- Journal Information:
- J. Immunol.; (United States), Vol. 136
- Country of Publication:
- United States
- Language:
- English
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