Splenic B cells and antigen-specific B cells process anti-Ig in a similar manner

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(89)90015-4· OSTI ID:5765568

Myers, C D; Vitetta, E S

B lymphocytes can process and present antigen to T cells. However, the fate of native antigen after its binding to specific B cells, i.e., the intracellular events involved in the processing and recycling of the antigenic fragments to the cell surface for antigen presentation, are not well understood. In the present study, we demonstrate that murine B cells degrade anti-Ig molecules bound to their surface and release acid soluble fragments into the supernatant. We also demonstrate that the kinetics of this process are identical for anti-mu, anti-delta, and anti-light chain antibodies, indicating that both surface IgM and surface IgD are equally effective in binding antigen and directing its processing. We also describe the effects of azide, chloroquine, and irradiation on this process. To extend these studies to the processing of specifically bound antigen, we demonstrate that highly purified trinitrophenyl antigen-binding cells degrade anti-Ig molecules with the same kinetics as unpurified splenic B cells. Thus, this purified population provides a suitable model system for the analysis of antigen degradation by antigen-specific cells.

• Research Organization: Univ. of Texas Southwestern Medical Center, Dallas (USA)
• OSTI ID: 5765568
• Journal Information: Cell. Immunol.; (United States), Vol. 121:1
• Country of Publication: United States
• Language: English

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