Study of the conditions of activation and stabilization of Shigella sonnei 47 DNA methylases in the process of fractionation, purification, and during storage
A comparative investigation was made of the factors of activation and conditions of stabilization of partially purified and individual fractions of DNA methylases of the strain Shigella sonnei 47. The influence of the temperature system, glycerin, albumin, protease inhibitors, divalent metal ions, as well as the conditions of storage at the value of the isoelectric point of the protein on the activity of the enzymes of methylation was studied. It was shown that the effects of activating factors and levels of stabilization differ appreciably, depending on the degree of purification and the composition of the enzyme preparations. It was established that glycerin is ineffective as a stabilizing agent. It was shown that of all the divalent cations studied the only activator universal for the methylases of Shigella sonnei 47 is Ca/sup 2 +/ ions. A pronounced stabilizing effect of albumin on the activity of these DNA methylases was demonstrated. With the exception of one enzymatic fraction, the protease inhibitors have virtually no effect on the level of methylating activity of the preparations. The phenomenon of spontaneous fluctuations of the methylating activity of Shigella sonnei 47 enzyme preparations during storage is described.
- Research Organization:
- Scientific-Research Institute of Medical Enzymology, Moscow, USSR
- OSTI ID:
- 5721910
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:8; Other Information: Translated from Biokhimiya; 51: No. 8, 1369-1376(Aug 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
METHYLATION
METHYL TRANSFERASES
ENZYME ACTIVITY
METABOLIC ACTIVATION
RADIOENZYMATIC ASSAY
STABILIZATION
BIOLOGICAL EFFECTS
BUFFERS
CATIONS
COBALT COMPOUNDS
ENZYME INHIBITORS
FRACTIONATION
LABELLING
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
POTASSIUM COMPOUNDS
PROTEINS
PURIFICATION
SHIGELLA
SODIUM COMPOUNDS
STORAGE LIFE
TEMPERATURE EFFECTS
TRITIUM COMPOUNDS
ZINC COMPOUNDS
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
BACTERIA
CHARGED PARTICLES
CHEMICAL REACTIONS
ENZYMES
IONS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SEPARATION PROCESSES
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
550401* - Genetics- Tracer Techniques
550201 - Biochemistry- Tracer Techniques