Comparison of effluent toxicity results using Ceriodaphnia dubia cultured on several diets

Journal Article · Fri Oct 01 00:00:00 EDT 1993 · Environmental Toxicology and Chemistry; (United States)

DOI:https://doi.org/10.1897/1552-8618(1993)12[1945:COETRU]2.0.CO;2· OSTI ID:5692368

Norberg-King, T J; Schmidt, S ^[1]

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1. U.S. Environmental Protection Agency, Duluth, MN (United States). Environmental Research Lab.

Several diets have been proposed for Ceriodaphnia dubia, but no single diet has been universally accepted as optimal for toxicity testing. Although several diets for Ceriodaphnia dubia culturing and testing are commonly used, little or no data exist on whether toxicity varies with the diet. This study evaluated several combinations of yeast-Cerophyl-trout chow (YCT), Selenastrum capricornutum, and Selenastrum capricornutum-Cerophyl foods for routine culture performance and the sensitivity of the offspring in subsequent acute toxicity tests with effluents. Variations in the diets included use of a vitamin-fortified yeast added to the YCT, algae (Selenastrum capricornutum) grown in two different algal media, and different feeding rates of the algae-Cerophyl diets. Eleven diets were evaluated in a multigeneration feeding study, but only seven were used in subsequent toxicity tests. The young produced from each of the seven diets were tested in 48-h acute tests with three different effluents across the generations. Toxicity tests with the effluents gave LC50s that were within a factor of two of one another, regardless of the food used for culturing. These results indicate that several diets are satisfactory for culturing Ceriodaphnia dubia and that the results of the toxicity tests are comparable.

• OSTI ID: 5692368
• Journal Information: Environmental Toxicology and Chemistry; (United States), Vol. 12:10; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHEMICAL EFFLUENTS
TOXICITY
DAPHNIA
SENSITIVITY
COMPARATIVE EVALUATIONS
DIET
WATER POLLUTION
ANIMALS
AQUATIC ORGANISMS
ARTHROPODS
BRANCHIOPODS
CHEMICAL WASTES
CRUSTACEANS
EVALUATION
INVERTEBRATES
NONRADIOACTIVE WASTES
POLLUTION
WASTES
560300* - Chemicals Metabolism & Toxicology