Quantitative measurement of DNA strand breaks and repair in. gamma. -irradiated human leukocytes from normal and ataxia telangiectasia donors
Fluorimetric analysis of DNA unwinding, which allows measurement of DNA strand breaks in human leukocytes, has been optimized by reducing the amount of cells required for the test and by modifying the DNA alkali unwinding conditions. The permitted measurement of DNA strand-break induction in cells irradiated with low (0.5-7 Gy) or high doses (5-20 Gy) of ..gamma.. rays. Linear dose-response curves were obtained for both dose ranges. Presence of cysteamine during irradiation caused a decrease in the extent of DNA strand breaks. The kinetics of the DNA standard-break rejoining process appeared to be biphasic over the dose range of 2-20 Gy when plotted on a linear vs linear axis (percentage of damage as a function of time). No difference in the level of DNA strand breaks and the rate of repair of these breaks was observed between leukocytes from three ataxia telangiectasia patients and those from normal donors.
- Research Organization:
- Institut Curie, Paris, France
- OSTI ID:
- 5642457
- Journal Information:
- Radiat. Res.; (United States), Vol. 102:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
FLUORESCENCE SPECTROSCOPY
LEUKOCYTES
BIOLOGICAL RADIATION EFFECTS
MEA
RADIOSENSITIVITY EFFECTS
STRAND BREAKS
DNA REPAIR
CESIUM 137
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
TELANGIECTASIS
ALKALI METAL ISOTOPES
AMINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD
BLOOD CELLS
BODY FLUIDS
CESIUM ISOTOPES
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
IONIZING RADIATIONS
ISOTOPES
MATERIALS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RECOVERY
REPAIR
SKIN DISEASES
SPECTROSCOPY
THIOLS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)