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Title: Physiological characterization of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and. cap alpha. factor pheromones

Saccharomyces cerevisiae MATa cells carrying mutations in either sst1 or sst2 are supersensitive to the G1 arrest induced by ..cap alpha.. factor pheromone. When sst1 mutants were mixed with normal SST/sup +/ cells, the entire population recovered together from ..cap alpha.. factor arrest, suggesting that SST/sup +/ cells helped sst1 mutants to recover. Complementation tests and linkage analysis showed that sst1 and bar1, a mutation which eliminates the ability of MATa cells to act as a ''barrier'' to the diffusion of ..cap alpha.. factor, were lesions in the same genes. These findings suggest that sst1 mutants are defective in recovery from ..cap alpha.. factor arrest because they are unable to degrade the pheromone. In contrast, recovery of sst2 mutants was not potentiated by the presence of SST/sup +/ cells in mixing experiments. When either normal MATa cells or mutant cells carrying defects in sst1 or sst2 were exposed to ..cap alpha.. factor for 1 h and then washed free of the pheromone, the sst2 cells subsequently remained arrested in the absence of ..cap alpha.. factor for a much longer time than SST/sup +/ or sst1 cells. These observations suggest that the defect in sst2 mutants is intrinsic to the cellmore » and is involved in the mechanism of ..cap alpha.. factor action at some step after the initial interaction of the pheromone with the cell. The presence of an sst2 mutation appears to cause a growth debility, since repeated serial subculture of haploid sst2-1 strains led to the accumulation of faster-growing revertants that were pheromone resistant and were mating defective (''sterile'').« less
Authors:
;
Publication Date:
OSTI Identifier:
5606779
Resource Type:
Journal Article
Resource Relation:
Journal Name: Mol. Cell. Biol.; (United States); Journal Volume: 2:1
Research Org:
Dept. of Microbiology, Univ. of Cincinnati, College of Medicine, Cincinnati, OH 45267
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHEROMONE; GENE MUTATIONS; METABOLISM; STRUCTURE-ACTIVITY RELATIONSHIPS; SACCHAROMYCES CEREVISIAE; GENOME MUTATIONS; MOLECULAR BIOLOGY; MUTANTS; PHYSIOLOGY; BIOLOGICAL PATHWAYS; CELL DIFFERENTIATION; DIFFUSION; GENES; MITOSIS; PLANT CELLS; REPRODUCTION; TECHNOLOGY ASSESSMENT; CELL DIVISION; FUNGI; MICROORGANISMS; MUTATIONS; PLANTS; SACCHAROMYCES; SECRETION; YEASTS 550400* -- Genetics; 550500 -- Metabolism