Recombinant human fibrinogen and sulfation of the. gamma. prime chain
- Univ. of Washington, Seattle (United States)
- ZymoGenetics, Inc., Seattle, WA (United States)
Human fibrinogen and the homodimeric {gamma}{prime}-chain-containing variant have been expressed in BHK cells using cDNAs coding for the {alpha},{beta}, and {gamma} (or {gamma}{prime}) chains. The fibrinogens were secreted at levels greater than 4 {mu}g (mg of total cell protein){sup {minus}1}day{sup {minus}1} and were biologically active in clotting assays. Recombinant fibrinogen containing the {gamma}' chain incorporated {sup 35}SO{sub 4} into its chains during biosynthesis, while no incorporation occurred in the protein containing the {gamma} chain. The identity of the sulfated {gamma}{prime} chain was verified by its ability to form dimers during clotting. In addition, carboxypeptidase {Upsilon} digestion of the recombinant fibrinogen containing the {gamma}{prime} chain released 96% of the {sup 35}S label from the sulfated chain, and the radioactive material was identified as tyrosine O-sulfate. These results clarify previous findings of the sulfation of tyrosine in human fibrinogen.
- OSTI ID:
- 5603784
- Journal Information:
- Biochemistry; (United States), Vol. 30:39; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBOXYPEPTIDASES
ENZYME ACTIVITY
FIBRINOGEN
BIOSYNTHESIS
CYSTEINE
LIVER CELLS
MAN
RECOMBINANT DNA
SULFATES
SULFUR 35
TYROSINE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
THIOLS
VERTEBRATES
550200* - Biochemistry