Euglena mitochondria and chloroplasts form tyrosine-O-sulfate

Journal Article · Sat Apr 01 04:00:00 UTC 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6611025

Saidha, T; Hanfstingl, U; Schiff, J A ^[1]

---

1. Brandeis Univ., Waltham, MA (USA)

Mitochondria from light-grown wild-type Euglena gracilis var. bacillaris Cori or dark-grown mutant W{sub 10}BSmL incubated with {sup 35}SO{sub 4}{sup 2{minus}} and ATP, or with {sup 14}C-tyrosine, non-radioactive sulfate and ATP accumulate a labeled compound in the medium. Since this compound shows exact coelectrophoresis with tyrosine-O-sulfate (TOS) at pH 2.0, 5.8 or 8.0., yields sulfate and tyrosine on acid hydrolysis, and treatment with aryl sulfatase from Aerobacter aerogenes yields sulfate and tyrosine but no tyrosine methyl ester, it is identified as TOS. No TOS is found outside purified developing chloroplasts incubated with {sup 35}SO{sub 4}{sup 2{minus}} and ATP, but both chloroplasts and mitochondria form to {sup 35}S externally when incubated with adenosine 3{prime} phosphate 5{prime}phospho({sup 35}S) sulfate (PAP{sup 35}S). Since no tyrosine need be added, tyrosine is provided from endogenous sources. Although TOS is found in the free pool of Euglena cells it cannot be detected in proteins of cells or mucus ruling our sulfation of tyrosine of protein or incorporation of TOS into proteins. The system forming TOS is membrane-bound and may be involved in tyrosine transport.

• OSTI ID: 6611025
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
ALGAE
AMINO ACIDS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLOROPLASTS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTROPHORESIS
ENZYMES
EUGLENA
EUGLENOPHYCOTA
EVEN-ODD NUCLEI
HYDROLASES
HYDROLYSIS
HYDROXY ACIDS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LYSIS
MASTIGOPHORA
MEMBRANE TRANSPORT
MICROORGANISMS
MITOCHONDRIA
NUCLEI
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXYGEN COMPOUNDS
PLANTS
PROTOZOA
RADIOISOTOPES
SOLVOLYSIS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
TRACER TECHNIQUES
TYROSINE
UNICELLULAR ALGAE