Recombinant human fibrinogen and sulfation of the. gamma. prime chain

Journal Article · Tue Oct 01 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00103a004· OSTI ID:5603784

Farrell, D H; Huang, S; Chung, D W; Davie, E W ^[1]; Mulvihill, E R ^[2]

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1. Univ. of Washington, Seattle (United States)
2. ZymoGenetics, Inc., Seattle, WA (United States)

Human fibrinogen and the homodimeric {gamma}{prime}-chain-containing variant have been expressed in BHK cells using cDNAs coding for the {alpha},{beta}, and {gamma} (or {gamma}{prime}) chains. The fibrinogens were secreted at levels greater than 4 {mu}g (mg of total cell protein){sup {minus}1}day{sup {minus}1} and were biologically active in clotting assays. Recombinant fibrinogen containing the {gamma}' chain incorporated {sup 35}SO{sub 4} into its chains during biosynthesis, while no incorporation occurred in the protein containing the {gamma} chain. The identity of the sulfated {gamma}{prime} chain was verified by its ability to form dimers during clotting. In addition, carboxypeptidase {Upsilon} digestion of the recombinant fibrinogen containing the {gamma}{prime} chain released 96% of the {sup 35}S label from the sulfated chain, and the radioactive material was identified as tyrosine O-sulfate. These results clarify previous findings of the sulfation of tyrosine in human fibrinogen.

• OSTI ID: 5603784
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:39; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
CARBOXYPEPTIDASES
COAGULANTS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
FIBRINOGEN
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
LIVER CELLS
MAMMALS
MAN
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SOMATIC CELLS
SULFATES
SULFUR 35
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
THIOLS
TYROSINE
VERTEBRATES