Effect of counting errors on immunoassay precision

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Clinical Chemistry (Winston-Salem, North Carolina); (USA)

OSTI ID:5547377

Klee, G G; Post, G ^[1]

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1. Mayo Foundation, Rochester, MD (USA)

Using mathematical analysis and computer simulation, we studied the effect of gamma scintillation counting error on two radioimmunoassays (RIAs) and an immunoradiometric assay (IRMA). To analyze the propagation of the counting errors into the estimation of analyte concentration, we empirically derived parameters for logit-log data-reduction models for assays of digoxin and triiodothyronine (RIAs) and ferritin (IRMA). The component of the analytical error attributable to counting variability, when expressed as a CV of the analyte concentration, decreased approximately linearly with the inverse of the square root of the maximum counts bound. Larger counting-error CVs were found at lower concentrations for both RIAs and the IRMA. Substantially smaller CVs for overall assay were found when the maximum counts bound progressively increased from 500 to 10,000 counts, but further increases in maximum bound counts resulted in little decrease in overall assay CV except when very low concentrations of analyte were being measured. Therefore, RIA and IRMA systems based in duplicate determinations having at least 10,000 maximum counts bound should have adequate precision, except possibly at very low concentrations.

• OSTI ID: 5547377
• Journal Information: Clinical Chemistry (Winston-Salem, North Carolina); (USA), Vol. 35:7; ISSN 0009-9147
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIGOXIN
RADIOIMMUNOASSAY
FERRITIN
ACCURACY
TRIIODOTHYRONINE
COMPUTERIZED SIMULATION
DATA COVARIANCES
MAN
MONTE CARLO METHOD
SCINTILLATION COUNTING
ANIMALS
BIOASSAY
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
COMPLEXES
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIGITALIS GLYCOSIDES
DRUGS
GLYCOSIDES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
IRON COMPLEXES
ISOTOPE APPLICATIONS
MAMMALS
METALLOPROTEINS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
SIMULATION
THYROID HORMONES
TRACER TECHNIQUES
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques