Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.16.5894· OSTI ID:5517468

Kim, Yongkyu; Wells, S; Lau, Yunfai Chris; Lee, A S ^[1]

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1. Univ. of Southern California School of Medicine, Los Angeles (USA)

Recent evidence on the transcriptional regulation of the human thymidine kinase (TK) gene raises the possibility that cell-cycle regulatory sequences may be localized within its promoter. A hybrid gene that combines the TK 5{prime} flanking sequence and the coding region of the bacterial neomycin-resistance gene (neo) has been constructed. Upon transfection into a hamster fibroblast cell line K12, the hybrid gene exhibits cell-cycle-dependent expression. Deletion analysis reveals that the region important for cell-cycle regulation is within {minus}441 to {minus}63 nucleotides from the transcriptional initiation site. This region ({minus}441 to {minus}63) also confers cell-cycle regulation to the herpes simplex virus thymidine kinase (HSVtk) promoter, which is not expressed in a cell-cycle manner. The authors conclude that the {minus}441 to {minus}63 sequence within the human TK promoter is important for cell-cycle-dependent expression.

• OSTI ID: 5517468
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:16; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL CYCLE
GENE REGULATION
GENE REPRESSORS
DNA SEQUENCING
PHOSPHOTRANSFERASES
TRANSCRIPTION
GENES
HERPES SIMPLEX
PHOSPHORUS 32
PLASMIDS
THYMIDINE
TRITIUM COMPOUNDS
VIRUSES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SKIN DISEASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VIRAL DISEASES
550201* - Biochemistry- Tracer Techniques