Identification of G sub 1 -S-phase-regulated region in the human thymidine kinase gene promoter

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.7.3834· OSTI ID:6511270

Roehl, H H; Conrad, S E ^[1]

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1. Michigan State Univ., East Lansing, MI (USA). Dept. of Microbiology

The authors have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G{sub 1}-S-phase regulation to the linked {ital neo} gene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G{sub 1}- and S-phase cells and in the various deletion mutants tested.

• OSTI ID: 6511270
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:7; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
CELL CYCLE
CHROMOSOMAL ABERRATIONS
DRUGS
ENZYMES
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC MAPPING
HETEROCYCLIC COMPOUNDS
MAPPING
MUTANTS
MUTATIONS
NEOMYCIN
NUCLEOPROTEINS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
PYRIMIDINES
RIBOSIDES
THYMIDINE
TRANSCRIPTION
TRANSFERASES