Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.85.2.469· OSTI ID:5515232

Chappell, J; Nable, R

Large amounts of the sesquiterpenoid capsidiol accumulated in the media of tobacco (Nicotiana tabacum L. cv KY14) cell suspension cultures upon addition of fungal elicitor. Capsidiol accumulation was proportional to the amount of elicitor added. The accumulation of capsidiol was preceded by a transient increase in the capsidiol de novo synthesis rate as measured by the incorporation of exogenous (/sup 14/C)acetate. Changes in 3-hydroxy-3-methylglutaryl-CoA reductase activity, an enzyme of general isoprenoid metabolism, paralleled the changes in (/sup 14/C)acetate incorporation into capsidiol. Incubation of the cell cultures with mevinolin, a potent in vitro inhibitor of the tobacco HMGR enzyme activity, inhibited the elicitor-induced capsidiol accumulation in a concentration dependent manner. (/sup 14/C)Acetate incorporation into capsidiol was likewise inhibited by mevinolin treatment. Unexpectedly, (/sup 3/H) mevalonate incorporation into capsidiol was also partially inhibited by mevinolin, suggesting that mevinolin may effect secondary sites of sesquiterpenoid biosynthesis in vivo beyond HMGR. The data indicated the importance of the induced HMGR activity for capsidiol production in elicitor-treated tobacco cell suspension cultures.

• Research Organization: Univ. of Kentucky, Lexington, KY (United States)
• OSTI ID: 5515232
• Journal Information: Plant Physiol.; (United States), Vol. 85:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OXIDOREDUCTASES
ENZYME ACTIVITY
TERPENES
BIOSYNTHESIS
ACETATES
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
CELL CULTURES
ENZYME INHIBITORS
MEVALONIC ACID
NICOTIANA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
ENZYMES
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques