Fungal elicitor induces sesquiterpenoid biosynthesis in tobacco cell suspension cultures
Large amounts of the sesquiterpenoid capsidiol accumulate in the media of tobacco cell suspension cultures upon addition of fungal elicitor. The accumulation of capsidiol was preceded by a transient increase in the capsidiol de novo synthesis rate as measured by the incorporation of exogenous /sup 14/C-acetate or /sup 3/H-mevalonate. Changes in 3-hydroxy-3-methylglutaryl-CoA reductase activity (HMGR; ES 1.1.1.34), an enzyme of general isoprenoid metabolism, parallel those measured for the /sup 14/C-acetate incorporation rate into capsidol. Incubation of the cell cultures with mevinolin, a potent in vitro inhibitor of the tobacco HMGR enzyme activity, inhibits the elicitor-induced capsidiol accumulation. /sup 14/C-acetate incorporation into capsidiol by elicitor-induced cell culture was likewise inhibited by mevinolin treatment. These results implicate HMGR activity as a rate limiting step for elicitor-induced capsidiol production in tobacco cell suspension cultures.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 6020587
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Journal Name: Plant Physiol., Suppl.; (United States) Vol. 83:4; ISSN PPYSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBON 14 COMPOUNDS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
IMMUNE REACTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
SYNTHESIS
TERPENES
TOBACCO
TRACER TECHNIQUES
TRITIUM COMPOUNDS