Effects of chemical sympathectomy on mucus secretion by surface goblet cells from rat colon
The authors examined the effects of 6-hydroxydopamine (60HDA), the beta adrenergic agonist isoproterenol (ISO) and normal saline (NS) diluent on baseline mucus secretion from rat colonic goblet cells. Mucus secretion was evaluated by labeling mucin granules with /sup 3/H-glucosamine (10 ..mu..Ci/g BW). All rats were sacrificed at 2 or 4 hrs after administration of either 60HDA (100 mg/kg), ISO (100mg/kg) or NS. Samples of colon were fixed and processed for microscopy. Sections were mounted, stained, dipped in photographic emulsion and exposed for 3-5 wks. Rates of mucus synthesis and secretion were determined by scoring each favorably oriented surface goblet cell for the maximal apical translocation of labeled granules from cell base (1) to apical surface (7). Means and S.D. (N=50) were determined for each treatment group. Non-sympathectomized control rats treated with ISO showed a highly significant decrease in mucus secretion at both 2 and 8 hrs after dosing (p<0.001). Rats pretreated with 60HDA at time 0 and 5 days prior to sacrifice showed decreased mucus section at 2 hours and increased secretion at 4 hrs after dosing (p<0.001). A highly significant difference in mucus secretion was observed between 2 and 4 hr scores for the time 60HDA group. Results of this study strongly suggest that baseline synthesis/secretion rates in goblet cells are under sympathetic control in the colon.
- Research Organization:
- Louisiana State Univ. School of Medicine, Shreveport
- OSTI ID:
- 5509278
- Report Number(s):
- CONF-8604222-; TRN: 86-026539
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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DOPAMINE
BIOLOGICAL EFFECTS
MUCOUS MEMBRANES
SECRETION
SYMPATHOMIMETICS
ANIMAL CELLS
GLUCOSAMINE
LARGE INTESTINE
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
HEXOSAMINES
HEXOSES
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INTESTINES
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LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
RODENTS
SACCHARIDES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques