Biochemical and behavioral effects of phospholipase A/sub 2/ and morphine microinjections in the periaqueductal gray of the rat
In order to characterize the in vivo action of phospholipase A/sub 2/ (PLA/sub 2/) on opiate receptors and opiate-induced behaviors, the effects of injections of PLA/sub 2/ into the periaqueductal gray region (PAG) of the rat were assessed on free fatty acid (FFA) release, opiate-binding levels, and morphine-induced behaviors. Rats received bilateral PAG injections of 2 ..mu..g of PLA/sub 2/ while anesthetized. One hour later, regions around the cannulae tracts in PLA/sub 2/-treated rats contained over 2.5 times more FFA than saline-injected controls, and /sup 3/H-dihydromorphine binding was reduced on average more than 70%. In another series of experiments, conscious rats were given 2 ..mu..g of PLA/sub 2/ prior to 10 ..mu..g of morphine through cannulae chronically implanted into the PAG. PLA/sub 2/ did not significantly attenuate morphine-induced analgesia as measured by the tail-flick test to radiant heat, but did prevent the explosive motor behavior observed following morphine injections alone. PLA/sub 2/ by itself did not induce analgesia, but did cause explosive motor behavior 2 hr after the injections. Neither lysophosphatidylcholine nor trypsin resulted in motor seizures following PAG injections. It was concluded that the behavioral effects of PLA/sub 2/ result from the unique properties of the enzyme, rather than generalized membrane damage, and that the opioid sites and mechanisms that mediate analgesia are different from those associated with explosive motor behavior. 36 references, 2 figures, 2 tables.
- Research Organization:
- Univ. of Rochester, NY
- OSTI ID:
- 5416300
- Journal Information:
- Life Sci.; (United States), Vol. 36:6
- Country of Publication:
- United States
- Language:
- English
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CHOLINE
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RECEPTORS
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550201* - Biochemistry- Tracer Techniques