NMR imaging and spectroscopy of the mammalian central nervous system after heavy ion radiation

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577182· OSTI ID:5406570

Richards, T; Budinger, T F

NMR imaging, NMR spectroscopy, and histopathologic techniques were used to study the proton relaxation time and related biochemical changes in the rodent brain after in vivo helium beam irradiation with single doses of 10, 20, 30, and 50 Gy. Two-dimensional Fourier transform spin-echo imaging and saturation recovery with projection reconstruction were used to measure the NMR relaxation parameters. These parameters were correlated with proton spectroscopy and histopathology. Additional high resolution in vitro proton spectroscopy was performed on brain extracts to observe chemical changes that could not be seen in vivo. The major findings from these experiments were that at 4-14 days postirradiation, image intensity and T1 relaxation time decreased on the irradiated side and increased on the nonirradiated side relative to nonirradiated control animals. In vivo surface coil proton spectroscopy methods demonstrated changes in lipid and phosphatidylcholine (p-choline) peaks. In vitro studies of the aqueous fraction of brain extracts showed radiation-induced changes in lactate, 4-aminobutyric acid, and p-choline peak areas. In the organic fraction, radiation-induced changes were observed in phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine. With histology and Evans blue injections, blood-brain barrier alterations were seen as early as 4 days after a dose of 50 Gy.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• OSTI ID: 5406570
• Journal Information: Radiat. Res.; (United States), Vol. 113:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD-BRAIN BARRIER
PATHOLOGICAL CHANGES
BRAIN
BIOLOGICAL RADIATION EFFECTS
NMR IMAGING
NMR SPECTRA
CHEMICAL COMPOSITION
ELECTRON SPIN RESONANCE
HELIUM IONS
HISTOLOGICAL TECHNIQUES
ION BEAMS
LIPIDS
NUCLEAR MAGNETIC RESONANCE
PROTONS
RATS
ANIMALS
BARYONS
BEAMS
BIOLOGICAL EFFECTS
BODY
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
DIAGNOSTIC TECHNIQUES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
IONS
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RESONANCE
RODENTS
SPECTRA
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550600 - Medicine