Human HST1 (HSTF1) gene maps to chromosome band 11q13 and coamplifies with the INT2 gene in human cancer

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.13.4861· OSTI ID:5404658

Yoshida, Michihiro C; Wada, Makio; Satoh, Hitoshi; Yoshida, Teruhiko; Sakamoto, Hiromi; Miyagawa, Kiyoshi; Yokota, Jun; Koda, Toshiaki; Kakinuma, Mitsuaki; Sugimura, Takashi; Terada, Masaaki ^[1]

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1. Hokkaido Univ., Sapporo (Japan)

The human HST1 gene, previously designated the hst gene, and now assigned the name HSTF1 for heparin-binding secretory transforming factor in human gene nomenclature, was originally identified as a transforming gene in DNAs from human stomach cancers by transfection assay with mouse NIH 3T3 cells. The amino acid sequence of the product deduced from DNA sequences of the HST1 cDNA and genomic clones had approximately 40% homology to human basic and acidic fibroblast growth factors and mouse Int-2-encoded protein. The authors have mapped the human HST1 gene to chromosome 11 at band q13.3 by Southern blot hybridization analysis of a panel of human and mouse somatic cell hybrids and in situ hybridization with an HST1 cDNA probe. The HST1 gene was found to be amplified in DNAs obtained from a stomach cancer and a vulvar carcinoma cell line, A431. In all of these samples of DNA, the INT2 gene, previously mapped to human chromosome 11q13, was also amplified to the same degree as the HST1 gene.

• OSTI ID: 5404658
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:13; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
CELL TRANSFORMATIONS
DNA
GENE AMPLIFICATION
MAN
NEOPLASMS
PHOSPHORUS 32
STOMACH
TUMOR CELLS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
GASTROINTESTINAL TRACT
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
VERTEBRATES
550401* - Genetics- Tracer Techniques