Visualization of INT2 and HST1 amplification in oral squamous cell carcinoma

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:133536

Lese, C M; Gollin, S M; Rossie, K M ^[1]

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1. Univ. of Pittsburgh and Pittsburgh Cancer Institute, PA (United States); and others

Oral squamous cell carcinoma (OSCC) is the sixth most frequent cancer worldwide. Amplification of band 11q13, which includes the INT2/FGF3, HST1/FGF4, BCL1, PRAD1/CCND1, and EMS1 genes, is frequent in head and neck squamous cell carcinoma. We characterized seven OSCC cell lines by classical and molecular cytogenetic analysis. Corresponding freshly dissociated tumor and adjacent margin tissue from three of these (PCI:SCC029, 030, and 044) were analyzed by molecular cytogenetics. We observed homogeneously staining regions (hsrs), usually thought to be the sites of gene amplification, in four of seven cell lines (003, 016, 029, and 044). In 029 and 044, the hsrs were localized to 11q13 on an apparently deleted 11. 016 had an hsr at 11q13 and 003 had two hsrs, one at 11q23 and the other on a marker chromosome. To determine whether INT2 and HST1 were amplified in the aforementioned fresh tissues and cell lines from our patients and to confirm the chromosomal location of the amplification, we used dual color fluorescence in situ hybridization (FISH) with DNA probes to these genes and the chromosome 11 centromere (from Oncor, Inc.). Coamplification of INT and HST1 was detected at the hsr sites in the tumor cell cultures from 003, 016, 029, and 044 and in the freshly dissociated tumor tissue from 029 and 044 (fresh tissue from 003 and 016 is not available). No amplification was seen in the other fresh tumor cells, cell lines, or adjacent oral mucosa corresponding to the hsr-positive or negative tumors. The presence of coamplified INT2 and HST1 in the direct and cultured tumor cells suggests that the amplification was present in the patient and not simply due to karyotypic evolution in vitro. Therefore, 11q13 amplification may play a key role in the development and/or progression of OSCC.

• OSTI ID: 133536
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CARCINOMAS
CENTROMERES
CHROMOSOMAL ABERRATIONS
DNA HYBRIDIZATION
FLUORESCENCE
GENE AMPLIFICATION
GENES
GENETIC MAPPING
HEAD
HUMAN CHROMOSOMES
KARYOTYPE
NECK
PROBES