Polyclonal activation of rat B cells. I. A single mitogenic signal can stimulate proliferation, but three signals are required for differentiation. [Salmonella typhimurium]
A water-soluble, proteinaceous preparation derived from the cell walls of Salmonella typhimurium Re mutants has recently been tested in this laboratory for its ability to act as a mitogen for rat lymphocytes. This preparation (STM) has been found to be a potent simulator of B lymphocyte proliferation, as measured both by /sup 3/H-TdR incorporation and by cell cycle analysis performed with flow cytofluorometry. STM stimulates approximately 50% of rat B cells to enter cycle. Previous investigations by others have shown that at least two sets of signals are required for B cell differentiation; (a) proliferation signals that may consist of both a stimulator of B cell conversion from G/sub 0/ to G/sub 1/ and growth factors, and (b) differentiation signals that probably include at least two B cell differentiation factors (BCDF). When STM was tested in a differentiation system it did not drive purified B cells to differentiate to PFC, either alone or when supplemented with a supernatant from concanavalin A-stimulated spleen cells (CAS). However, when both CAS and dextran sulfate (DXS) were supplied to the STM-stimulated cells, a large number of PFC resulted. DXT does not act by stimulating an additional, CAS-responsive B cell subset, since it has only a marginal effect upon /sup 3/H-TdR uptake and does not increase the number of B cells in cycle when used together with STM. The authors that the two agents may be acting sequentially: STM stimulates the B cells to proliferate, and DXS drives the proliferating cells to become responsive to CAS. This suggests that the signals for B cell differentiation must consist of at least three activities: a trigger to stimulate the cells to proliferate, a factor to drive the cells to a BCDF-responsive state, and a BCDF that can drive the cells to secrete antibody.
- Research Organization:
- Univ. of Iowa, Iowa City
- OSTI ID:
- 5321582
- Journal Information:
- J. Immunol.; (United States), Vol. 136:11
- Country of Publication:
- United States
- Language:
- English
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CELL PROLIFERATION
STIMULATION
LYMPHOCYTES
CELL DIFFERENTIATION
MITOGENS
BIOLOGICAL EFFECTS
ANTIBODIES
CELL CYCLE
CELL FLOW SYSTEMS
CONCANAVALIN
RATS
SALMONELLA TYPHIMURIUM
SPLEEN CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AGGLUTININS
ANIMAL CELLS
ANIMALS
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
HEMAGGLUTININS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LECTINS
LEUKOCYTES
MAMMALS
MATERIALS
MICROORGANISMS
RODENTS
SALMONELLA
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques