Developmental regulation of early mouse embryogenesis. A. Onset of insulin and related growth factor binding. B. Expression of selected proto-oncogenes

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5242003

Mattson, B A

Experimental and clinical evidence suggest a role for insulin and related growth factors in fetal growth. It was therefore of interest to determine the onset of insulin binding to cells of early embryos. These studies are the first to demonstrate stage-specific insulin binding during mouse preimplantation embryogenesis. Insulin binding was detected at the morula, blastocyst, and blastocyst outgrowth stages using indirect immunofluorescence and light microscopic autoradiographic techniques. No evidence of insulin binding was seen on unfertilized oocytes, two-, four-, or eight-cell embryos. Results of competitive inhibition studies using unlabeled insulin-like growth factors (IGFs) suggest binding of /sup 125/I-insulin to type I IGF receptors as well. Further studies are needed to confirm the presence of an IGF receptor in preimplantation embryos.

• Research Organization: Philadelphia Coll. of Pharmacy and Science, PA (USA)
• OSTI ID: 5242003
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
INSULIN
MICE
ONTOGENESIS
AUTORADIOGRAPHY
EMBRYOS
IODINE 125
ANIMALS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques