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Functional roles of insulin and insulin-like growth factors in preimplantation mouse embryo development

Journal Article · · In Vitro Cellular and Developmental Biology; (USA)
DOI:https://doi.org/10.1007/BF02624438· OSTI ID:6062552
; ;  [1]
  1. Albert Einstein Medical Center, Philadelphia, PA (USA)
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Growth factors are known to play important roles in cellular proliferation and differentiation. However, little information is available concerning their roles in the earliest stages of mammalian development. The effect of physiologic levels of insulin, insulinlike growth factor-I, and insulinlike growth factor II (IGF-I and -II) on DNA, RNA, and protein synthesis in preimplantation stages of the mouse are described in this study. Quantitative studies of the incorporation of labeled thymidine, uridine, and methionine into trichloroacetic acid-insoluble material by different developmental stages of preimplantation mouse embryos labeled in vitro, indicate that physiologic levels of insulin stimulated DNA, RNA, and protein synthesis with significant effects observed first at the morula stage of development. In contrast, neither IGF-I nor IGF-II stimulated DNA, RNA, or protein synthesis to a significant degree under the same experimental conditions. These results suggest a functional role for insulin at the earliest stages of mammalian embryogenesis.
OSTI ID:
6062552
Journal Information:
In Vitro Cellular and Developmental Biology; (USA), Journal Name: In Vitro Cellular and Developmental Biology; (USA) Vol. 26:11; ISSN 0883-8364; ISSN ICDBE
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
AZINES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
CARBOXYLIC ACIDS
DNA
DRUGS
FUNCTIONS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INSULIN
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
METHIONINE
MICE
MITOGENS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ONTOGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PROTEINS
PYRIMIDINES
RIBOSIDES
RNA
RODENTS
SULFUR ISOTOPES
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE
VERTEBRATES