Developmental regulation of early mouse embryogenesis. A. Onset of insulin and related growth factor binding. B. Expression of selected proto-oncogenes
Thesis/Dissertation
·
OSTI ID:5242003
Experimental and clinical evidence suggest a role for insulin and related growth factors in fetal growth. It was therefore of interest to determine the onset of insulin binding to cells of early embryos. These studies are the first to demonstrate stage-specific insulin binding during mouse preimplantation embryogenesis. Insulin binding was detected at the morula, blastocyst, and blastocyst outgrowth stages using indirect immunofluorescence and light microscopic autoradiographic techniques. No evidence of insulin binding was seen on unfertilized oocytes, two-, four-, or eight-cell embryos. Results of competitive inhibition studies using unlabeled insulin-like growth factors (IGFs) suggest binding of /sup 125/I-insulin to type I IGF receptors as well. Further studies are needed to confirm the presence of an IGF receptor in preimplantation embryos.
- Research Organization:
- Philadelphia Coll. of Pharmacy and Science, PA (USA)
- OSTI ID:
- 5242003
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
GROWTH FACTORS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EMBRYOS
GROWTH FACTORS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ONTOGENESIS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RODENTS
VERTEBRATES