Rat pulmonary alveolar macrophages in-vitro cytotoxicity to six metal dusts. Technical report, May-August 1985

Technical Report · Tue Dec 01 00:00:00 EST 1987

OSTI ID:5234424

Anderson, R S; Gutshall, L L; Thomson, S A

Pulmonary alveolar macrophages (PAMs) were harvested from rats by bronchopulmonary lavage and exposed in vitro to aqueous suspensions of six metal dusts: aluminum, silicated aluminum, carbon, graphite, titanium dioxide, and silica. The LC50 value for each dust was calculated by logarithmic linear regression analysis of the toxicity data from the experimental exposure concentrations. Cytotoxicity was determined by metal concentration-dependent inability of PAMs to exclude the trypan blue dye from their cytroplasm. After exposure, PAMs usually bore numerous bound metal particles; but phagocytosis was rare, probably because the cells were not activated in situ prior to removal from the lungs.

• Research Organization: Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (USA)
• OSTI ID: 5234424
• Report Number(s): AD-A-189040/9/XAB; CRDEC-TR-88037
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
54 ENVIRONMENTAL SCIENCES
DUSTS
PARTICULATES
METALS
TOXICITY
PHAGOCYTOSIS
RETICULOENDOTHELIAL SYSTEM
CARBON
GRAPHITE
MACROPHAGES
PHAGOCYTES
PROGRESS REPORT
SILICA
SILICATES
ANIMAL CELLS
ANIMAL TISSUES
BODY
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DOCUMENT TYPES
ELEMENTAL MINERALS
ELEMENTS
MINERALS
NONMETALS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PARTICLES
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
TISSUES
550900* - Pathology
500200 - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)