Vitamin B12 assays compared by use of patients sera with low vitamin B12 content

Journal Article · Wed May 01 00:00:00 EDT 1985 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5229411

Sheridan, B L; Pearce, L C

The authors compared four radioisotope dilution (RD) methods and a microbiological assay for measuring concentrations of vitamin B12 in a selected panel of serum samples from patients known to be deficient in the vitamin. Low (less than 100 ng/L) and borderline (100-180 ng/L) results were similar between methods, but use of the manufacturers recommended ranges for borderline results would have changed the diagnostic classifications for 22 of 38 samples. Results of all the RD methods inter-correlated well, but less so with the microbiological assay. Borderline, nondiagnostic results were common to all methods, and no apparent advantage was gained from using the microbiological assay.

• Research Organization: Victoria General Hospital, Halifax, Nova Scotia
• OSTI ID: 5229411
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIAGNOSTIC TECHNIQUES
COMPARATIVE EVALUATIONS
VITAMIN B-12
NUTRITIONAL DEFICIENCY
BIOASSAY
DIAGNOSIS
ISOTOPE DILUTION
PATIENTS
DRUGS
HEMATINICS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
TRACER TECHNIQUES
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques