Monoclonal antibodies for the detection of trace chemicals

Conference · Fri Aug 15 00:00:00 EDT 1986

OSTI ID:5217714

Vanderlaan, M; Van Emon, J; Watkins, B; Stanker, L

Problems in analytical chemistry may limit monitoring for trace organic residues by traditional chromatographic methods. For example, the cost and analysis time per sample may preclude adequate sampling, making the development of alternative technologies desirable. Immunoassays are one such alternative, with the potential for cost reduction by automation and parallel sample processing. A particularly significant advance in the past decade has been the development of monoclonal antibodies, which offer greater selectivity and reproducibility than conventional antisera. Immunoassays can be developed that use simple, field-portable instrumentation, give rapid results, and have detection limits of less than a part-per-billion. This paper reviews the general technology for developing monoclonal antibodies to small organic molecules using the immunoassay of 2,3,7,8-tetrachlorodibenzodioxin (2,3,7,8-TCDD) as an example. 18 refs., 2 figs., 1 tab.

• Research Organization: Lawrence Livermore National Lab., CA (USA). Biomedical Sciences Div.
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 5217714
• Report Number(s): UCRL-95278; CONF-8608117-1; ON: DE86016001
• Resource Relation: Conference: 6. international congress of pesticide chemistry, Ottawa, Canada, 10 Aug 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DIOXIN
BIOASSAY
HYBRIDOMAS
MONOCLONAL ANTIBODIES
STRUCTURE-ACTIVITY RELATIONSHIPS
ANIMAL CELLS
ANTIBODIES
HETEROCYCLIC COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
560300* - Chemicals Metabolism & Toxicology