Convenient preparative synthesis of ( sup 14 C)trehalose from ( sup 14 C)glucose by intact Escherichia coli cells

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Applied and Environmental Microbiology; (USA)

OSTI ID:5159662

Brand, B; Boos, W ^[1]

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1. Univ. of Konstanz (Germany, F.R.)

At high osmolarity, Escherichia coli synthesizes trehalose intracellularly, irrespective of the nature of the carbon source. Synthesis proceeds via the transfer of UDP-glucose to glucose 6-phosphate, yielding trehalose 6-phosphate, followed by its dephosphorylation to trehalose. This reaction was exploited to preparatively synthesize ({sup 14}C)trehalose from exogenous ({sup 14}C)glucose by using intact bacteria of a mutant (DF214) that could not metabolize glucose. The total yield of radiochemically pure trehalose from glucose was routinely more than 50%.

• OSTI ID: 5159662
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 55:9; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
ESCHERICHIA COLI
TRACER TECHNIQUES
ALDEHYDES
BACTERIA
CARBOHYDRATES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
550501* - Metabolism- Tracer Techniques