Changes in the sup 14 C-labeled cell wall components with chase time after incorporation of UDP( sup 14 C)glucose by intact cotton fibers
- Univ. of California, Riverside (USA)
Intact, in vitro-grown cotton fibers will incorporate ({sup 14}C)glucose from externally supplied UDP({sup 14}C)glucose into a variety of cell wall components including cellulose; this labeled fraction will continue to increase up to 4 hours chase time. In the fraction soluble in hot water there was no significant change in total label; however, the largest fraction after the 30 minute pulse with UDP({sup 14}C)glucose was chloroform-methanol soluble (70%) and showed a significant decrease with chase. The lipids that make up about 85% of this fraction were identified by TLC as steryl glucosides, acylated steryl glucosides, and glucosyl-phosphoryl-polyprenol. Following the pulse, the loss of label from acylated steryl glucosides and glucosylphophoryl-polyprenol was almost complete within 2 hours of chase; steryl glucosides made up about 85% of the fraction at that chase time. The total loss in the lipid fraction (about 100 picomoles per milligram dry weight of fiber) with chase times of 4 hours approximates the total gain in the total glucans.
- OSTI ID:
- 6937728
- Journal Information:
- Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 86:4; ISSN PLPHA; ISSN 0032-0889
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL WALL
CHROMATOGRAPHY
COTTON
FIBERS
GLUCOSE
GLYCOSIDES
HEXOSES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MONOSACCHARIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHYSIOLOGY
REACTION KINETICS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
UDPG