Cell cycle expression of RNA duplex unwindase activity in mammalian cells
Journal Article
·
· Mol. Cell. Biol.; (United States)
An RNA duplex unwindase activity has been found by using an in vitro assay with various types of mammalian, somatic cells, including HeLa, mouse plasmacytoma, and Burkitt lymphoma. The unwindase activity is very low in mouse fibroblast 3T3 cells arrested into quiescence, but increases when the cells are released into renewed growth by serum. In addition, a gel retardation assay proved to be specific and sensitive for detection of RNA duplex-unwindase complexes.
- Research Organization:
- The Wistar Institute, 36th Street at Spruce, Philadelphia, PA 19104 (US)
- OSTI ID:
- 5115211
- Journal Information:
- Mol. Cell. Biol.; (United States), Vol. 8:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
RNA
STRUCTURE-ACTIVITY RELATIONSHIPS
CELL CYCLE
CELL DIVISION
ENZYME ACTIVITY
FIBROBLASTS
HELA CELLS
IN VITRO
LYMPHOMAS
PHENOTYPE
SOMATIC CELLS
ANIMAL CELLS
CONNECTIVE TISSUE CELLS
DISEASES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
550200* - Biochemistry
550300 - Cytology
RNA
STRUCTURE-ACTIVITY RELATIONSHIPS
CELL CYCLE
CELL DIVISION
ENZYME ACTIVITY
FIBROBLASTS
HELA CELLS
IN VITRO
LYMPHOMAS
PHENOTYPE
SOMATIC CELLS
ANIMAL CELLS
CONNECTIVE TISSUE CELLS
DISEASES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
550200* - Biochemistry
550300 - Cytology