Anomalous cell surface structure of sickle cell anemia erythrocytes as demonstrated by cell surface labeling and endo-beta-galactosidase treatment
Erythrocyte surface glycoproteins from patients with various types of sickle cell anemia have been analyzed and compared with those from normal individuals. By hemagglutination with various anti-carbohydrate antibodies, sickle cells showed profound increase of i antigens and moderate increase of GlcNAc beta 1 leads to 3Gal beta 1 leads to 3 Glc structure, whereas antigenicity toward globosidic structure was unchanged. In parallel to these findings, erythrocytes of sickle cell patients have additional sialylated lactosaminoglycan in Band 3. Thus, it can be concluded that erythrocytes of sickle cell patients are characterized by an altered cell surface structure which does not appear to be due to topographical changes of cell surface membrane. It is possible that the anemia or the ''stress'' hematopoiesis in these patients is responsible for these changes.
- Research Organization:
- Department of Biochemical Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA
- OSTI ID:
- 5105535
- Journal Information:
- J. Supramol. Struct. Cell. Biochem.; (United States), Vol. 17:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ERYTHROCYTES
SURFACE PROPERTIES
GALACTOSIDASE
BIOCHEMISTRY
GLUCOPROTEINS
SICKLE CELL ANEMIA
ANTIGEN-ANTIBODY REACTIONS
BOROHYDRIDES
CELL MEMBRANES
ELECTROPHORESIS
PATIENTS
ANEMIAS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BORON COMPOUNDS
CARBOHYDRATES
CELL CONSTITUENTS
CHEMISTRY
DISEASES
ENZYMES
GLYCOSYL HYDROLASES
HEMIC DISEASES
HYDROGEN COMPOUNDS
HYDROLASES
MATERIALS
MEMBRANES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PROTEINS
SACCHARIDES
SYMPTOMS
550300* - Cytology
550900 - Pathology