Modeling sickle cell vasoocculsion in the rat leg: Quantification of trapped sickle cells and correlation with sup 31 P metabolic and sup 1 H magnetic resonance imaging changes

Journal Article · Mon May 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.10.3808· OSTI ID:5299064

Fabry, M E; Rajanayagam, V; Fine, E; Holland, S; Gore, J C; Nagel, R L; Kaul, D K ^[1]

---

1. Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY (USA)

The authors have developed an animal model to elucidate the acute effects of perfusion abnormalities on muscle metabolism induced by different density-defined classes of erythrocytes isolated from sickle cell anemia patients. Technetium-99m ({sup 99m}Tc)-labeled, saline-washed normal (AA), homozygous sickle (SS), or high-density SS (SS4) erythrocytes were injected into the femoral artery of the rat and quantitative {sup 99m}Tc imaging, {sup 31}P magnetic resonance spectroscopy by surface coil at 2 teslas, and {sup 1}H magnetic resonance imaging at 0.15 tesla were performed. Between 5 and 25 {mu}l of SS4 cells was trapped in the microcirculation of the thigh. In contrast, fewer SS discocytes (SS2) or AA cells were trapped. After injection of SS4 cells an initial increase in inorganic phosphate was observed in the region of the thigh served by the femoral artery, intracellular pH decreased, and subsequently the proton relaxation time T{sub 1} reached a broad maximum at 18-28 hr. When T{sub 1} obtained at this time was plotted against the volume of cells trapped, an increase of T{sub 1} over the control value of 411 {plus minus} 48 msec was found that was proportional to the number of cells trapped. They conclude that the densest SS cells are most effective at producing vasoocclusion. The extent of the change detected by {sup 1}H magnetic resonance imaging is dependent on the amount of cells trapped in the microcirculation and the magnitude of the initial increase of inorganic phosphate.

• OSTI ID: 5299064
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:10; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD FLOW
RADIOISOTOPE SCANNING
ERYTHROCYTES
NUCLEAR MAGNETIC RESONANCE
SICKLE CELL ANEMIA
PATHOLOGICAL CHANGES
ARTERIES
BIOLOGICAL MODELS
ISOMERIC NUCLEI
MUSCLES
NMR SPECTRA
PATIENTS
PERFUSED TISSUES
PHOSPHORUS 31
PROTONS
TECHNETIUM 99
ANEMIAS
ANIMAL TISSUES
BARYONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
COUNTING TECHNIQUES
DISEASES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEMIC DISEASES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATERIALS
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RESONANCE
SPECTRA
STABLE ISOTOPES
SYMPTOMS
TECHNETIUM ISOTOPES
TISSUES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics