Quantitative assessment of rabbit alveolar macrophage function by chemiluminescence
Rabbit alveolar macrophages (RAM) were cultured for 24 hr with concentrations ranging from 3 to 12 ..mu..g/ml of vanadium oxide (V/sub 2/O/sub 5/), a known cytotoxic agent, or with high-molecular-weight organic by-products from coal gasification processes. After culture the cells were harvested and tested for functional capacity using three types of indicators: (1) luminol-amplified chemiluminescence (CL), which quantitatively detects photon emission due to respiratory burst activity measured in a newly designed instrument with standardized reagents; (2) the reduction of nitro blue tetrazolium-saturated polyacrylamide beads, a semiquantitative measure of respiratory burst activity; and (3) phagocytic efficiency, defined as percentage of cells incorporating immunoglobulin-coated polyacrylamide beads. Chemiluminescence declined linearly with increasing concentrations of V/sub 2/O/sub 5/ over the dose range tested. Dye reduction and phagocytic efficiency similarly decreased with increasing V/sub 2/O/sub 5/ concentration, but were less sensitive indicators of functional impairment than CL as measured by the amount required to reduce the response to 50% of untreated cells. The effect of coal gasification condensates on RAM function varied, but in general these test also indicated that the CL response was the most sensitive indicator.
- Research Organization:
- Packard Instrument Co., Downers Grove, IL
- DOE Contract Number:
- W-31-109-ENG-38
- OSTI ID:
- 5093556
- Journal Information:
- Environ. Res.; (United States), Vol. 37:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
COMBUSTION PRODUCTS
TOXICITY
MACROPHAGES
BIOLOGICAL FUNCTIONS
VANADIUM OXIDES
CELL CULTURES
CHEMILUMINESCENCE
COAL GASIFICATION
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
MEASURING METHODS
PHAGOCYTOSIS
RABBITS
ANIMAL CELLS
ANIMALS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DATA
FUNCTIONS
GASIFICATION
INFORMATION
LUMINESCENCE
MAMMALS
NUMERICAL DATA
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
SOMATIC CELLS
THERMOCHEMICAL PROCESSES
TRANSITION ELEMENT COMPOUNDS
VANADIUM COMPOUNDS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)