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Title: Effects of OK-432 on murine bone marrow and the production of natural killer cells

The streptococcal preparation, OK-432, which augments anti-tumor responses in humans and mice, has been shown to be a potent immunomodulator. Among its effects is a pronounced augmentation of natural killer (NK) activity. The hypothesis that OK-432 alters the rates of production and maturation of NK cells in the bone marrow was tested. Studies to determine the kinetic parameters of NK cell production in normal C57BL/6J mice using tritiated thymidine, /sup 3/H-TdR, as a DNA marker are described. We are now extending those studies to determine the effect of OK-432 on the bone marrow and on the production of NK cells in the marrow. Initial observations are reported which indicate that OK-432 has profound effects on the cellularity and mitotic activity of the bone marrow, and in particular, on cells with the characteristics of natural killer cells within the marrow. 17 refs., 3 figs., 4 tabs.
Authors:
;
Publication Date:
OSTI Identifier:
5072424
Report Number(s):
DOE/EV/10270-T7
ON: DE87002046
DOE Contract Number:
AT06-79EV10270
Resource Type:
Technical Report
Resource Relation:
Other Information: Microfiche only, copy does not permit paper copy reproduction
Research Org:
Washington Univ., Seattle (USA). Dept. of Biological Structure
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BONE MARROW CELLS; CELL DIFFERENTIATION; ENDOTOXINS; BIOLOGICAL FUNCTIONS; LEUKOCYTES; ANTINEOPLASTIC DRUGS; MICE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; ANTIGENS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DRUGS; FUNCTIONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; RODENTS; SOMATIC CELLS; TOXIC MATERIALS; TOXINS; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques; 550301 -- Cytology-- Tracer Techniques