Effects of OK-432 on murine bone marrow and the production of natural killer cells

Technical Report · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5072424

Pollack, S B; Rosse, C

The streptococcal preparation, OK-432, which augments anti-tumor responses in humans and mice, has been shown to be a potent immunomodulator. Among its effects is a pronounced augmentation of natural killer (NK) activity. The hypothesis that OK-432 alters the rates of production and maturation of NK cells in the bone marrow was tested. Studies to determine the kinetic parameters of NK cell production in normal C57BL/6J mice using tritiated thymidine, /sup 3/H-TdR, as a DNA marker are described. We are now extending those studies to determine the effect of OK-432 on the bone marrow and on the production of NK cells in the marrow. Initial observations are reported which indicate that OK-432 has profound effects on the cellularity and mitotic activity of the bone marrow, and in particular, on cells with the characteristics of natural killer cells within the marrow. 17 refs., 3 figs., 4 tabs.

• Research Organization: Washington Univ., Seattle (USA). Dept. of Biological Structure
• DOE Contract Number: AT06-79EV10270
• OSTI ID: 5072424
• Report Number(s): DOE/EV/10270-T7; ON: DE87002046
• Resource Relation: Other Information: Microfiche only, copy does not permit paper copy reproduction
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
CELL DIFFERENTIATION
ENDOTOXINS
BIOLOGICAL FUNCTIONS
LEUKOCYTES
ANTINEOPLASTIC DRUGS
MICE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
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BODY FLUIDS
CONNECTIVE TISSUE CELLS
DRUGS
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
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RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
VERTEBRATES
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550301 - Cytology- Tracer Techniques