Milk progesterone radioimmunoassay using radioiodinated tracers: a rapid and reliable assay system

Journal Article · Sat Sep 01 00:00:00 EDT 1984 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(84)90003-5· OSTI ID:5023224

Espinosa, J; Botana, L M; Puentes, E; Regueiro, B J

Both the validity and practicability of a direct progesterone radioimmunoassay based on radioiodinated progesterone tracers were studied. The results obtained show the reliability of the assay; when compared with assays based on /sup 3/H-progesterone tracers there are fewer steps for assay execution, saving time and reducing the number of reagents used. Various commercially available /sup 125/I-progesterone tracers were assayed, and only those with an 11 alpha-hemisuccinate bridge were suitably bound by antisera raised against progesterone-bovine serum albumin conjugates having identical bridge structure. The bridge effect caused no observable alteration in validity parameters. Finally, the results support the utility of this assay as a practical method of early diagnosis of pregnancy and as a reliable experimental technique to monitor cow ovarian function.

• Research Organization: Univ. of Santiago, Santiago de Compostela, Spain
• OSTI ID: 5023224
• Journal Information: Steroids; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PREGNANCY
DIAGNOSIS
PROGESTERONE
RADIOIMMUNOASSAY
ALBUMINS
CATTLE
IODINE 125
LABELLED COMPOUNDS
MILK
OVARIES
RELIABILITY
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
FOOD
GONADS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PROTEINS
RADIOASSAY
RADIOISOTOPES
RUMINANTS
STEROID HORMONES
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques