Transmission of a Filterable Agent from Rat Leukaemia Induced by X-Ray Irradiation and Treatment with Methylcholanthrene
Leukemia was induced in rats by combination of x irradiation and oral application of methylcholanthrene. The rats were irradiated by a single dose of 800 r, and methylcholanthrene was applied 3 times a week by stomach tube in a dose of 1 mg for 9 months. From 60 rats, myelogenous leukemia developed in 2 and lymphatic leukemia in 1. The myelobiastic leukemia proved to be transplantable and was maintained as MR-leukemia. After irtravenous injection of 1 to 10 x 10/ sup 6/ leukemic cells, obtained from the liver and spleen, the disease developed in adult rats in 6 to 10 days. As early as the 2nd or 3rd day after inoculation, leukemic infiltration of organs, especially liver and spleen, were seen. The rats died exhibiting signs of generalized leukemia within 10 days. If cell-free filtrates from the liver and spleen of rats bearing MR leukemia were injected into newborn and 4-week-old rats, myelogenous leukemia developed in the newborn group in 24% after a latency period of 520 days and in 33% of the 4-week-old group after 570 days, on an average. The induced leukemias were transplantable into both suckling and adult rats. Many of the injected animals, which did not develop leukemia, died of cirrhosis of the liver. The results suggest that the leukemia induced by irradiation and chemical carcinogen might be caused by a submicroscopic virus-like agent.
- Research Organization:
- Oncological Research Inst., Bratislava
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-010258
- OSTI ID:
- 4715143
- Journal Information:
- Acta Haematologica, Vol. 26, Issue 4; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0001-5792
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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