Clinical progression of transplanted large granular lymphocytic leukemia in Fischer 334 rats exposed to 60 Hz magnetic fields

Journal Article · Tue Jan 19 00:00:00 EST 1999 · Bioelectromagnetics

DOI:https://doi.org/10.1002/(SICI)1521-186X(1999)20:1<48::AID-BEM7>3.0.CO;2-8· OSTI ID:15003093

Morris, James E ^[1]; Sasser, Lyle B ^[1]; Miller, Douglas L ^[1]; Dagle, Gerald E ^[2]; Rafferty, C N ^[3]; Ebi, K L ^[3]; Anderson, Larry E ^[1]

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1. BATTELLE (PACIFIC NW LAB)
2. WASHINGTON STATE UNIV TC
3. Electric Power Research Institute

The purpose of this study was to determine if 60 Hz magnetic fields could alter the clinical progression of leukemia in an animal model. Large granular lymphocytic (LGL) leukemia cells from spleens of leukemic rats were transplanted into young male Fischer rats, producing signs of leukemia in about 2-3 months. The animals were injected with 2.2 x 107 LGL leukemia cells at the initiation of the study and assigned to 4 treatment groups 108/group) as follows: (1) 10 G linearly polarized 60 Hz magnetic fields, (2) sham exposed null energized unit with residual 20 mG fields, (3) ambient controls < 1 mG, and (4) positive controls (a single 5 Gy whole body exposure to 60Co 4 days prior to initiation of exposure). The magnetic fields were activated 20h/day, 7 days/week. Eighteen Rats (18 from each treatment group) were bled, killed, and evaluated at a5, 6, 7, 8, 9, and 11 weeks of exposure. Hematological endpoints, changes in spleen growth, and LGL cell infiltration into the spleen and liver were measured to evaluate the leukemia progression. Significant differences were not detected between the magnetic field exposed groups and the ambient control group, although the clinical progress of leukemia was enhanced in the positive control animals. These data indicate that exposure to sinusoidal, linearly polarized 60 Hz, 10 G magnetic fields did not significantly alter the clinical progression of LGL leukemia. Furthermore, the data are in general agreement with previous results of a companion repeated-bleeding study.

• Research Organization: Pacific Northwest National Lab., Richland, WA (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 15003093
• Report Number(s): PNWD-SA-4596; TRN: US200420%%248
• Journal Information: Bioelectromagnetics, Vol. 20, Issue 1; Other Information: PBD: 19 Jan 1999
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RATS
LEUKEMIA
LIVER
MAGNETIC FIELDS
MALES
SPLEEN
HZ RANGE