The ZNF75 zinc finger gene subfamily: Isolation and mapping of the four members in humans and great apes
- Consiglio Nazionale delle Ricerche, Milan (Italy); and others
We have previously reported the characterization of the human ZNF75 gene located on Xq26, which has only limited homology (less than 65%) to other ZF genes in the databases. Here, we describe three human zinc finger genes with 86 to 95% homology to ZNF75 at the nucleotide level, which represent all the members of the human ZNF75 subfamily. One of these, ZNF75B, is a pseudogene mapped to chromosome 12q13. The other two, ZNF75A and ZNF75C, maintain on ORF in the sequenced region, and at least the latter is expressed in the U937 cell line. They were mapped to chromosomes 16 and 11, respectively. All these genes are conserved in chimpanzees, gorillas, and orangutans. The ZNF75B homologue is a pseudogene in all three great apes, and in chimpanzee it is located on chromosome 10 (phylogenetic XII), at p13 (corresponding to the human 12q13). The chimpanzee homologue of ZNF75 is also located on the Xq26 chromosome, in the same region, as detected by in situ hybridization. As expected, nucleotide changes were clearly more abundant between human and organutan than between human and chimpanzee or gorilla homologues. Members of the same class were more similar to each other than to the other homologues within the same species. This suggests that the duplication and/or retrotranscription events occurred in a common ancestor long before great ape speciation. This, together with the existance of at least two genes in cows and horses, suggests a relatively high conservation of this gene family. 20 refs., 5 figs., 1 tab.
- OSTI ID:
- 469037
- Journal Information:
- Genomics, Vol. 35, Issue 2; Other Information: PBD: 15 Jul 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
HUMAN X CHROMOSOME
GENETIC MAPPING
PROTEINS
GENE REGULATION
TRANSCRIPTION
BIOLOGICAL EVOLUTION
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
HUMAN CHROMOSOME 12
HUMAN CHROMOSOME 16
HUMAN CHROMOSOMES
DNA HYBRIDIZATION
NUCLEOTIDES
PRIMATES
PROBES
POLYMERASE CHAIN REACTION