Isolation, cDNA, and genomic structure of a conserved gene (NOF) at chromosome 11q13 next to FAU and oriented in the opposite transcriptional orientation

Journal Article · Sat Jun 15 00:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0310· OSTI ID:466696

Kas, K; Meyen, E; Van De Ven, W J.M. ^[1]

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1. Univ. of Leuven & Flanders Interuniversity Institute for Biotechnology, Leuven (Belgium); and others

In our effort to characterize a gene at chromosome 11q13 involved in a t(11;17)(q13;q21) translocation in B-non-Hodgkin lymphoma, we have identified a novel human gene, NOF (Neighbour of FAU). It maps right next to FAU in a head to head configuration separated by a maximum of 146 nucleotides. cDNA clones representing NOF hybridized to a 2.2-kb mRNA present in all tissues tested. The largest open reading frame appeared to contain 166 amino acids and is proline rich, and the sequence shows no homology with any known gene in the public databases. The NOF gene consists of 4 exons and 3 introns spanning approximately 5 kb, and the boundaries between exons and introns follow the GT/AG rule. The NOF locus is conserved during evolution, with the predicted protein having over 80% identity to three translated mouse and rat ESTs of unknown function. Moreover, the mouse ESTs map in the same organization, closely linked to the FAU gene, in the mouse genome. NOF, however, is not affected by the t(11;17)(q13;121) chromosomal translocation. 14 refs., 2 figs.

• OSTI ID: 466696
• Journal Information: Genomics, Vol. 34, Issue 3; Other Information: PBD: 15 Jun 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
TRANSCRIPTION
GENETIC MAPPING
DNA SEQUENCING
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA-CLONING
BIOLOGICAL EVOLUTION
HUMAN CHROMOSOMES
CHROMOSOMAL ABERRATIONS
HUMAN CHROMOSOME 17
MAN
LYMPHOMAS
NUCLEOTIDES
DNA HYBRIDIZATION
AMINO ACID SEQUENCE
EXONS
INTRONS
FLUORESCENCE