Regulation of growth hormone receptor and binding protein expression in domestic species

Journal Article · Sat Dec 31 00:00:00 EST 1994 · Proceedings of the Society for Experimental Biology and Medicine

DOI:https://doi.org/10.3181/00379727-206-43741· OSTI ID:466615

Bingham, B; Oldham, E R; Baumbach, W R ^[1]

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1. American Cyanamid Company, Princeton, NJ (United States)

Growth hormone receptor (GHR) expression has been analyzed at the RNA level. In the rat, relative expression of the RNA species encoding the GHR and the GH-binding protein (GHBP) appears to be sensitive to endocrine status. Full-length GHR cDNA clones from ovine, porcine, and chicken were used as probes to investigate the existence of unique RNAs for GHBPs in these species. In the sheep and pig, only a single, {approximately}4.5-kb RNA is apparent. Although quite high levels of GH binding activity are found in pig serum, a variety of methods failed to isolate a separate GHBP message, suggesting that porcine GHBP is produced via a mechanism different from that which is known for rat. One class of chicken GHR cDNA, resulting from alternative use of a splice acceptor 17 bases upstream of the intron 6/exon 7 junction, is also presented. 24 refs., 2 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 466615
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine, Vol. 206; Other Information: PBD: 1994
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
STH
RECEPTORS
GENE REGULATION
TRANSCRIPTION
DNA-CLONING
SPLICING
TISSUE DISTRIBUTION
GENE MUTATIONS
STRUCTURE-ACTIVITY RELATIONSHIPS
PROTEINS
DNA SEQUENCING
LIVER
NUCLEOTIDES
SHEEP
SWINE
ENDOCRINE GLANDS
CHICKENS
RNA
INTRONS
EXONS
DOMESTIC ANIMALS
PROBES
POLYMERASE CHAIN REACTION
AMINO ACIDS