Regulation of the growth hormone (GH) receptor and GH-binding protein mRNA

Journal Article · Sat Dec 31 04:00:00 EST 1994 · Proceedings of the Society for Experimental Biology and Medicine

DOI:https://doi.org/10.3181/00379727-206-43755· OSTI ID:466622

Kaji, Hidesuke; Ohashi, Shin-Ichirou; Abe, Hiromi; Chihara, Kazuo ^[1]

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1. Kobe Univ. School of Medicine, Kobe (Japan)

In fasting rats, a transient increase in growth hormone-binding protein (GHBP) mRNA levels was observed after 1 day, in muscle, heart, and liver, but not in fat tissues. The liver GH receptor (GHR) mRNA level was significantly increased after 1 day (but not after 5 days) of bovine GH (bGH) treatment in fed rats. Both the liver GHR mRNA level and the net increment of plasma IGF-I markedly decreased after 5 days of bGH administration in fasting rats. These findings suggest that GHR and GHBP mRNAs in the liver are expressed in a different way and that the expression of GHBP mRNA is regulated differently between tissues, at least in rats. The results also suggest that refractoriness to GH in a sustained fasting state might be beneficial in preventing anabolic effects of GH. In humans, GHR mRNA in lymphocytes, from subjects with either GH-deficiency or acromegaly, could be detected by the reverse transcription-polymerase chain reaction method. In one patient with partial GH insensitivity, a heterozygous missense mutation (P561T) was identified in the cytoplasmic domain of GHR. 15 refs., 4 figs.

• Sponsoring Organization: USDOE
• OSTI ID: 466622
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine, Journal Name: Proceedings of the Society for Experimental Biology and Medicine Vol. 206; ISSN PSEBAA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANABOLISM
BIOLOGICAL FUNCTIONS
CLEAVAGE
DNA SEQUENCING
DNA-CLONING
FASTING
GENE MUTATIONS
GENE REGULATION
LIVER
LYMPHOCYTES
MESSENGER-RNA
METABOLIC DISEASES
NUCLEOTIDES
PATIENTS
POLYMERASE CHAIN REACTION
PROBES
PROTEINS
RATS
RECEPTORS
STH
TISSUE DISTRIBUTION