Regulation of growth hormone receptor and binding protein expression in domestic species

Journal Article · Sat Dec 31 04:00:00 EST 1994 · Proceedings of the Society for Experimental Biology and Medicine

DOI:https://doi.org/10.3181/00379727-206-43741· OSTI ID:466615

Bingham, B; Oldham, E R; Baumbach, W R ^[1]

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1. American Cyanamid Company, Princeton, NJ (United States)

Growth hormone receptor (GHR) expression has been analyzed at the RNA level. In the rat, relative expression of the RNA species encoding the GHR and the GH-binding protein (GHBP) appears to be sensitive to endocrine status. Full-length GHR cDNA clones from ovine, porcine, and chicken were used as probes to investigate the existence of unique RNAs for GHBPs in these species. In the sheep and pig, only a single, {approximately}4.5-kb RNA is apparent. Although quite high levels of GH binding activity are found in pig serum, a variety of methods failed to isolate a separate GHBP message, suggesting that porcine GHBP is produced via a mechanism different from that which is known for rat. One class of chicken GHR cDNA, resulting from alternative use of a splice acceptor 17 bases upstream of the intron 6/exon 7 junction, is also presented. 24 refs., 2 figs., 3 tabs.

• Sponsoring Organization: USDOE
• OSTI ID: 466615
• Journal Information: Proceedings of the Society for Experimental Biology and Medicine, Journal Name: Proceedings of the Society for Experimental Biology and Medicine Vol. 206; ISSN PSEBAA; ISSN 0037-9727
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACIDS
CHICKENS
DNA SEQUENCING
DNA-CLONING
DOMESTIC ANIMALS
ENDOCRINE GLANDS
EXONS
GENE MUTATIONS
GENE REGULATION
INTRONS
LIVER
NUCLEOTIDES
POLYMERASE CHAIN REACTION
PROBES
PROTEINS
RECEPTORS
RNA
SHEEP
SPLICING
STH
STRUCTURE-ACTIVITY RELATIONSHIPS
SWINE
TISSUE DISTRIBUTION
TRANSCRIPTION