Comparison of the response of bacterial luminescence and mitochondrial respiration to the effluent of an oil refinery

Journal Article · Mon Apr 01 00:00:00 EST 1996 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1002/etc.5620150414· OSTI ID:268030

Riisberg, M; Bratlie, E; Stenersen, J ^[1]

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1. Univ. of Oslo (Norway)

The effects of oil refinery effluents on rat mitochondrial respiration and on the luminescence of the bacterium Photobacterium phosphoreum were compared. Mitochondria from male Wistar rat livers were exposed to different concentrations of refinery effluents in a semiclosed 3-ml reaction vessel. Respiration was measured polarographically with an oxygen electrode. Effects on P. phosphoreum were measured by the standard test developed by Microbics. The mitochondrial method showed EC50s in the range from 1 to 7.5%, while Microtox gave EC50 in the range from 30 to 42%. The higher sensitivity of mitochondria may be exploited in the development of a sensitive biosensor for toxicity of oil refinery effluents.

• Sponsoring Organization: USDOE
• OSTI ID: 268030
• Journal Information: Environmental Toxicology and Chemistry, Vol. 15, Issue 4; Other Information: PBD: Apr 1996
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
PETROLEUM REFINERIES
ENVIRONMENTAL IMPACTS
PETROLEUM PRODUCTS
TOXICITY
PHOTOSYNTHETIC BACTERIA
LUMINESCENCE
MITOCHONDRIA
SENSITIVITY
CHEMICAL EFFLUENTS
COMPARATIVE EVALUATIONS
BIOLOGICAL INDICATORS
POLLUTION
RATS