Localization of an epithelial-specific receptor kinase (EDDR1) to chromosome 6q16
- John Radcliffe Hospital, Oxford (United Kingdom); and others
A protein receptor tyrosine kinase (EDDR1) has been isolated from a complementary DNA library of SROV-3, an epithelial ovarian cancer cell line. The primary structure of the predicted amino acid sequence of the protein shows a novel N-terminal region that has homology to a factor VIII-like domain. The C-terminal catalytic domain has all of the canonical sequence motifs of a receptor tyrosine kinase with homology to the TRK-2H protein (49%), which suggests that it is a type II receptor. It is expressed in epithelial cells of several tissues. To determine the chromosomal localization of the gene, somatic cell hybrids were analyzed by PCR amplification using oligonucleotide primers specific for EDDR1. Segregation was observed to a hybrid containing human chromosome 6. Cosmids for EDDR1 were isolated from a human chromosome 6 cosmid library and were shown by fluorescence in situ hybridization to map to 6q16. 22 refs., 4 figs.
- OSTI ID:
- 250176
- Journal Information:
- Genomics, Vol. 25, Issue 2; Other Information: PBD: 20 Jan 1995
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
GENES
GENETIC MAPPING
DNA SEQUENCING
HUMAN CHROMOSOME 6
RECEPTORS
TYROSINE
PHOSPHOTRANSFERASES
OVARIES
NEOPLASMS
PROTEINS
AMINO ACID SEQUENCE
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA HYBRIDIZATION
FLUORESCENCE
SOMATIC CELLS
POLYMERASE CHAIN REACTION
NUCLEOTIDES
COSMIDS