Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)

Journal Article · Fri Jan 20 04:00:00 EST 1995 · Genomics

DOI:https://doi.org/10.1016/0888-7543(95)80055-Q· OSTI ID:250167

Nakagawara, A; Liu, X G; Ikegaki, N ^[1]

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1. Children`s Hospital of Philadelphia, PA (United States); and others

There is increasing evidence that neurotrophins and their receptors play an important role in regulating development of both the central and the peripheral nervous systems. Human TRK-A (NTRK1) and TRK-C (NTRK3) have been cloned and sequenced, but only a truncated form of human TRK-B has been published. Therefore, we isolated complementary DNAs spanning the entire coding region of both human full-length and truncated forms of TRK-B from human brain cDNA libraries. Human full-length TRK-B codes for a protein of 822 amino acid residues. The putative mature peptide sequence is 49% homologous to human TRK-A and 55% full-length human TRK-C, with 40% amino acid identity among TRK-A, -B, and -C. Nine of 13 cysteine residues, 4 of 12 N-glycosylation sites in the extracellular domain, and 10 of 13 tyrosine residues in the intracellular domain are conserved among human TRK-A, -B, and -C. There is a cluster of 10 serine residues in the juxtamembrane region of TRK-B that is absent in TRK-A. Two major sizes of TRK-B transcripts were expressed in human brain. Northern blot analysis using probes specific for the extracellular or the tyrosine kinase domain revealed that the 9.5-kb band encodes the truncated from of TRK-B mRNA. By fluorescence in situ hybridization and somatic cell hybrid mapping, the human TRK-B gene was localized to chromosome 9q22.1. 31 refs., 4 figs.

• OSTI ID: 250167
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 25; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 9
PEPTIDES
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RECEPTORS
SOMATIC CELLS
TRANSCRIPTION
TYROSINE