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Title: Diffusion studies on permeable nitroxyl spin probes through bilayer lipid membranes: A low frequency ESR study

Electron spin resonance (ESR) studies were carried out for permeable 2mM {sup 14}N-labeled deutrated 3 Methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) in pure water and 1mM, 2mM, 3mM, 4mM concentration of 14N-labeled deutrated MC-PROXYL in 400mM concentration of liposomal solution by using a 300 MHz ESR spectrometer. The ESR parameters such as linewidth, hyperfine coupling constant, g-factor, partition parameter and permeability were reported for these samples. The line broadening was observed for the nitroxyl spin probe in the liposomal solution. The line broadening indicates that the high viscous nature of the liposomal solution. The partition parameter and permeability values indicate the maximum diffusion of nitroxyl spin probes in the bilayer lipid membranes at 2 mM concentration of nitroxyl radical. This study illustrates that ESR can be used to differentiate between the intra and extra- membrane water by loading the liposome vesicles with a lipid-permeable nitroxyl spin probe. From the ESR results, the spin probe concentration was optimized as 2mM in liposomal solution for ESR phantom studies/imaging, invivo and invitro experiments.
Authors:
;  [1] ; ; ;  [2] ;  [3] ;  [4]
  1. Department of Physics, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)
  2. Department of Bio-functional Science, Kyushu University, Fukuoka (Japan)
  3. Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka (Japan)
  4. Department of Chemistry, NMSSVN College, Nagamalai, Madurai-625019, Tamilnadu (India)
Publication Date:
OSTI Identifier:
22490217
Resource Type:
Journal Article
Resource Relation:
Journal Name: AIP Conference Proceedings; Journal Volume: 1665; Journal Issue: 1; Conference: 59. DAE solid state physics symposium 2014, Tamilnadu (India), 16-20 Dec 2014; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CARBONYLS; CONCENTRATION RATIO; COUPLING CONSTANTS; DIFFUSION; ELECTRON SPIN RESONANCE; LANDE FACTOR; LAYERS; LINE BROADENING; LINE WIDTHS; LIPIDS; MEMBRANES; MHZ RANGE; NITROGEN 14; NITROXYL RADICALS; PERMEABILITY; PYRROLIDINES