Permeability studies of redox-sensitive nitroxyl spin probes in corn oil using an L-band ESR spectrometer

Journal Article · Mon May 23 04:00:00 EDT 2016 · AIP Conference Proceedings

DOI:https://doi.org/10.1063/1.4947650· OSTI ID:22606254

Jebaraj, D. David ^[1]; Utsumi, Hideo ^[2]; Asath, R. Mohamed; Benial, A. Milton Franklin, ^[3]

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1. Department of Physics, The American College, Madurai-625 002, Tamilnadu (India)
2. Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka 812-8582 (Japan)
3. Department of Physics, NMSSVN College, Madurai-625 019, Tamilnadu (India)

Electron spin resonance (ESR) studies were carried out for 2mM {sup 14}N labeled {sup 2}H enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL) and 3–carboxy-2,2,5,5,-tetramethyl-1-pyrrolidinyloxy (carboxy-PROXYL) in pure water and various concentrations of corn oil. The ESR parameters, such as the line width, hyperfine coupling constant, g-factor, rotational correlation time, partition parameter and permeability were reported for the samples. The line width broadening was observed for both nitroxyl radicals in corn oil solutions. The partition parameter for permeable MC-PROXYL in corn oil increases with increasing concentration of corn oil, which reveals that the nitroxyl spin probe permeates into the oil phase. From the results, the corn oil concentration was optimized as 50 % for phantom studies. The rotational correlation time also increases with increasing concentration of corn oil. The permeable and impermeable nature of nitroxyl spin probes was demonstrated. These results will be useful for the development of ESR/OMR imaging modalities in in vivo and in vitro studies.

• OSTI ID: 22606254
• Journal Information: AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1731; ISSN APCPCS; ISSN 0094-243X
• Country of Publication: United States
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CONCENTRATION RATIO
CORN OIL
CORRELATIONS
COUPLING CONSTANTS
DEUTERIUM
ELECTRON SPIN RESONANCE
ELECTRONS
LANDE FACTOR
LINE WIDTHS
NITROGEN 14
NITROXYL RADICALS
PERMEABILITY
PHANTOMS
PYRROLIDINES
SPECTROMETERS
SPIN
WATER