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Title: Construction and direct electrochemistry of orientation controlled laccase electrode

Highlights: • A recombinant laccase with Cys-6×His tag at the N or C terminus was generated. • Orientation controlled laccase electrodes were constructed via self assembly. • The electrochemical behavior of laccase electrodes was orientation dependent. • The C terminus tagged laccase was better for bioelectrocatalytic reduction of O{sub 2}. - Abstract: A laccase has multiple redox centres. Chemisorption of laccases on a gold electrode through a polypeptide tag introduced at the protein surface provides an isotropic orientation of laccases on the Au surface, which allows the orientation dependent study of the direct electrochemistry of laccase. In this paper, using genetic engineering technology, two forms of recombinant laccase which has Cys-6×His tag at the N or C terminus were generated. Via the Au-S linkage, the recombinant laccase was assembled orientationally on gold electrode. A direct electron transfer and a bioelectrocatalytic activity toward oxygen reduction were observed on the two orientation controlled laccase electrodes, but their electrochemical behaviors were found to be quite different. The orientation of laccase on the gold electrode affects both the electron transfer pathway and the electron transfer efficiency of O{sub 2} reduction. The present study is helpful not only to the in-depth understanding of the directmore » electrochemistry of laccase, but also to the development of laccase-based biofuel cells.« less
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [2]
  1. Key Laboratory of Colloid and Interface Chemistry of the Education Ministry of China, Shandong University, Jinan 250100 (China)
  2. State Key Laboratory of Microbial Technology of China, Shandong University, Jinan 250100 (China)
  3. (China)
Publication Date:
OSTI Identifier:
22416337
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 446; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; BIOFUELS; CARBON; CHEMISORPTION; EFFICIENCY; ELECTROCHEMISTRY; ELECTRODES; ELECTRON TRANSFER; GENETIC ENGINEERING; GOLD; NITROGEN; OXYGEN; POLYPEPTIDES; SURFACES