Lung Irradiation Increases Mortality After Influenza A Virus Challenge Occurring Late After Exposure

Journal Article · Wed May 01 00:00:00 EDT 2013 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/J.IJROBP.2012.10.019· OSTI ID:22224454

Manning, Casey M. ^[1]; Johnston, Carl J. ^[1]; Reed, Christina K. ^[2]; Lawrence, B. Paige ^[1]; Williams, Jacqueline P. ^[3]

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1. Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York (United States)
2. Department of Pediatrics, University of Rochester Medical Center, Rochester, New York (United States)
3. Department of Radiation Oncology, University of Rochester Medical Center, Rochester, New York (United States)

Purpose: To address whether irradiation-induced changes in the lung environment alter responses to a viral challenge delivered late after exposure but before the appearance of late lung radiation injury. Methods and Materials: C57BL/6J mice received either lung alone or combined lung and whole-body irradiation (0-15 Gy). At 10 weeks after irradiation, animals were infected with 120 HAU influenza virus strain A/HKx31. Innate and adaptive immune cell recruitment was determined using flow cytometry. Cytokine and chemokine production and protein leakage into the lung after infection were assessed. Results: Prior irradiation led to a dose-dependent failure to regain body weight after infection and exacerbated mortality, but it did not affect virus-specific immune responses or virus clearance. Surviving irradiated animals displayed a persistent increase in total protein in bronchoalveolar lavage fluid and edema. Conclusions: Lung irradiation increased susceptibility to death after infection with influenza virus and impaired the ability to complete recovery. This altered response does not seem to be due to a radiation effect on the immune response, but it may possibly be an effect on epithelial repair.

• OSTI ID: 22224454
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 86, Issue 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
CLEARANCE
DEATH
EDEMA
INFLUENZA
INFLUENZA VIRUSES
LUNGS
MICE
MORTALITY
RADIATION DOSES
RADIATION INJURIES
WHOLE-BODY IRRADIATION