Development of a Porcine Delayed Wound-Healing Model and Its Use in Testing a Novel Cell-Based Therapy

Journal Article · Mon Nov 01 00:00:00 EDT 2010 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/j.ijrobp.2010.05.002· OSTI ID:21451161

Hadad, Ivan ^[1]; Johnstone, Brian H ^[2]; Brabham, Jeffrey G ^[3]; Blanton, Matthew W ^[1]; Rogers, Pamela I; Fellers, Cory; Solomon, James L; Merfeld-Clauss, Stephanie ^[2]; DesRosiers, Colleen M; Dynlacht, Joseph R ^[3]; Coleman, John J ^[4]; March, Keith L ^[2]

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1. Department of Surgery, Indiana University School of Medicine, Indianapolis, IN (United States)
2. Indiana Center for Vascular Biology and Medicine, Indianapolis, IN (United States)
3. Division of Radiation Oncology, Indiana University School of Medicine, Indianapolis, IN (United States)
4. Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN (United States)

Purpose: A delayed full-thickness wound-healing model was developed and used for examining the capacity of adipose-derived stem cells (ASCs), either alone or in platelet-rich fibrin gels, to promote healing. Methods and Materials: Four pigs received electron beam radiation to the dorsal skin surface. Five weeks after radiation, subcutaneous fat was harvested from nonirradiated areas and processed to yield ASCs. Two weeks later, 28 to 30 full-thickness 1.5-cm{sup 2} wounds were made in irradiated and nonirradiated skin. Wounds were treated with either saline solution, ASCs in saline solution, platelet-rich plasma (PRP) fibrin gel, ASCs in PRP, or non-autologous green fluorescence protein-labeled ASCs. Results: The single radiation dose produced a significant loss of dermal microvasculature density (75%) by 7 weeks. There was a significant difference in the rate of healing between irradiated and nonirradiated skin treated with saline solution. The ASCs in PRP-treated wounds exhibited a significant 11.2% improvement in wound healing compared with saline solution. Enhancement was dependent on the combination of ASCs and PRP, because neither ASCs nor PRP alone had an effect. Conclusions: We have created a model that simulates the clinically relevant late radiation effects of delayed wound healing. Using this model, we showed that a combination of ASCs and PRP improves the healing rates of perfusion-depleted tissues, possibly through enhancing local levels of growth factors.

• OSTI ID: 21451161
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 78, Issue 3; Other Information: DOI: 10.1016/j.ijrobp.2010.05.002; PII: S0360-3016(10)00636-X; Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; ISSN 0360-3016
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ELECTRON BEAMS
FIBRIN
GELS
GROWTH FACTORS
HEALING
RADIATION DOSES
RADIATION INJURIES
STEM CELLS
SWINE
THERAPY
WOUNDS
ANIMAL CELLS
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BLOOD COAGULATION FACTORS
COLLOIDS
DISEASES
DISPERSIONS
DOMESTIC ANIMALS
DOSES
INJURIES
LEPTON BEAMS
MAMMALS
MEDICINE
MITOGENS
ORGANIC COMPOUNDS
PARTICLE BEAMS
PROTEINS
RADIATION EFFECTS
SCLEROPROTEINS
SOMATIC CELLS
VERTEBRATES